#Leica acquire software how to
#Leica acquire software software

The export function (right click on the file of interest) allows you to use your data stacks to create new files in different formats: Infinite numbers of cropped files can be created, and will be numbered accordingly (“Crop002”, “Crop003”, etc.). The oldest files are at the top and the most recently generated files at the bottom.įor example, editing a z-stack (as in generating a new z-stack file from part of the original stack) produces a new file in the folder that is named after the original, and includes “Crop001” in the name. The files are always listed top to bottom by their ages, NOT their names. These actions also generate new sub-files off the original. The “Process” tab (just to the right of the “Acquire” tab) allows you to do many useful things to your data files, such as cropping Z or T stacks, mosaic merging of a tile scan to produce a combined image, and Dye Separation. The original xyz files used to create the snapshots remain. Note that a snapshot has a different icon on the left (a multicolor page), “Snapshot” is included in the name, and it is an xy format file. The snapshot file will show up in the experiments tab: For example, when you right-click on the images in right side display window, you have an option to generate a snapshot: Multiplying the filesĪny kind of processing will generate new files. Terms like genotypes, treatments (or lack thereof), tissue type, and antibodies/markers used will help you keep your scans in order. You should also rename each scan, especially before you do any sort of processing on them. Writing those names down in your lab notebook will save you much time and anxiety. lif file and you are trying to find it on one of our computers, do NOT enter search terms based on names of individual scans. It CANNOT see inside and find individual scan names. the folder icon at the top of the list) is visible to a computer search function.

lif files- only the name of the experiment/project (i.e.

#Leica acquire software software

If you include forbidden symbols, the software will scold you and revert back to the old name.Ī very important fact about.

Your initials/name, the date of the scans, and shorthand identifying the specimens are good choices. Choose names that will help you find/recognize the experiments quickly. Right-click on the folder to open a new panel. One of the best ways to keep track of data is to name your new folders and scans as soon as you create them. If you accidentally start a scan with the wrong folder active, you can drag and drop that file into the desired folder after the scan is finished. Once scans are added, the combined size of all the files will be indicated (like the top folder, with 812.4 MB worth of scans). It has a (0) on the right to indicate that it is empty of any saved scans. In this example the “Experiment001” folder is active. You can have multiple folders present in the tab, but only one can be active at a time. The red highlighting indicates an active folder, i.e., that’s where any further scans and processing of scans will be sent. In the above example it contains one XYZ scan. The experiment is represented as a folder, which can contain multiple scans, and any processing of those scans. You can use the “Open” button to bring up an existing folder if you wish to add new scans and/or process existing scans. Doing a live scan will also generate a new folder. You can generate a new experiment folder by clicking the “New” button at the bottom of the panel. When you first start the LAS-AF software, the panel will be blank. It is located to the left of the Acquisition Tab at the top left of the Leica panel: The Experiment Tab allows the user to access and process individual scans. This can cause the files to multiply faster than fruit flies, so this section will help you keep track of and organize those files. Instead the software will generate copies with your alterations. One important principle to remember is that no matter how you edit/ process a file, your original data stacks will remain, unless you command the system to delete them. Understanding the system and how it is organized is important for keeping track of all your data. The Leica software allows you to quickly generate scans and do many sorts of processing on them.

Newer versions of the software now call it the “Project” Tab.

#Leica acquire software how to

The Leica “Experiment” Tab, or How to keep your Leica scans in order